Automated hot solvent extraction and HPLC determination of atrazine and its degradation products in soil

Citation
A. Hrdlicka et J. Dolinova, Automated hot solvent extraction and HPLC determination of atrazine and its degradation products in soil, J LIQ CHR R, 24(5), 2001, pp. 721-734
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
5
Year of publication
2001
Pages
721 - 734
Database
ISI
SICI code
1082-6076(2001)24:5<721:AHSEAH>2.0.ZU;2-1
Abstract
Automated hot solvent extraction of atrazine (ATR), desethylatrazine (DEA), and desisopropylatrazine (DIA) from soil samples has been optimized and va riation of recoveries with total organic carbon content (TOC) in soil has b een studied. Soil (20 g) was extracted for 30 min at an enhanced temperature with 120 mL of dichlormethane/acetone 65:35 v/v and, subsequently, rinsed 10 min with condensate. Extract was cleaned-up by gel permeation chromatography and an aliquot of triazine fraction was injected onto a narrow bore HPLC column pa cked with polymeric octadecyl phase. Mobile phase containing acetonitrile a nd 0.002 mol/L phosphate buffer of pH 6.8 was used for gradient elution. Recoveries higher than 98%, 93%, and 81% can be attained for ATR, DEA, and DIA (1 mug/g level), respectively, by extracting soils with TOC less than o r equal to 2.5%. With TOC increasing to 39% recovery, values decrease up to approx. 25%. Limits of detection based on a signal-to-noise ratio of 3 and calculated on a dry-weight basis were 4 ng/g ATR and 2 ng/g DEA or DIA. Re lative standard deviation of the measurement was 3.4% for ATR, 1.1% for DEA , and 3.6% for DIA. The method has been used for determination of atrazine and its degradation products in soil from maize fields.