Honey carbohydrate analysis by HPLC, with electrochemical detection, usinga Ni-Cr alloy electrode

Citation
Mi. Mora et Jm. Marioli, Honey carbohydrate analysis by HPLC, with electrochemical detection, usinga Ni-Cr alloy electrode, J LIQ CHR R, 24(5), 2001, pp. 711-720
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
5
Year of publication
2001
Pages
711 - 720
Database
ISI
SICI code
1082-6076(2001)24:5<711:HCABHW>2.0.ZU;2-O
Abstract
Honey carbohydrates were analyzed by high performance anion exchange liquid chromatography with constant potential amperometric detection at a nickel- chromium (80:20) alloy electrode. The sugars are oxidized by a surface cata lyzed process proposed to involve Ni(II)/Ni(III) oxyhydroxides, which are f ormed at approximately 0.500 V (Saturated Calomel as reference electrode). The honey content of glucose, fructose, and sucrose is determined in less t han 15 minutes per sample. The high sensibility achieved with this alloy electrode allowed for the ana lysis of disaccharides and oligosaccharides usually present in honey as min or components. The analysis of these carbohydrates may be useful for the de termination of the floral origin of honey.