Honey carbohydrates were analyzed by high performance anion exchange liquid
chromatography with constant potential amperometric detection at a nickel-
chromium (80:20) alloy electrode. The sugars are oxidized by a surface cata
lyzed process proposed to involve Ni(II)/Ni(III) oxyhydroxides, which are f
ormed at approximately 0.500 V (Saturated Calomel as reference electrode).
The honey content of glucose, fructose, and sucrose is determined in less t
han 15 minutes per sample.
The high sensibility achieved with this alloy electrode allowed for the ana
lysis of disaccharides and oligosaccharides usually present in honey as min
or components. The analysis of these carbohydrates may be useful for the de
termination of the floral origin of honey.