Screening for corticosteroids in topical pharmaceuticals by HPLC with a scanning ultraviolet detector

Authors
Citation
Jc. Reepmeyer, Screening for corticosteroids in topical pharmaceuticals by HPLC with a scanning ultraviolet detector, J LIQ CHR R, 24(5), 2001, pp. 693-709
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
5
Year of publication
2001
Pages
693 - 709
Database
ISI
SICI code
1082-6076(2001)24:5<693:SFCITP>2.0.ZU;2-2
Abstract
A screening procedure was developed for the detection of 49 corticosteroids in topical pharmaceutical products. These products were analyzed by revers ed-phase HPLC using two gradient systems: a linear gradient of 18-82% aceto nitrile over 12 min and a linear gradient of 38-88% methanol over 12 min. A lthough all corticosteroids were not resolved during a single 12-min gradie nt analysis, a peak retention time from a sample analysis was usually suffi cient to limit an unknown to 1-3 probable corticosteroids. Steroids unresol ved with the first gradient system were resolved with the second one, with the exception of three steroid pairs. Assignments based on retention times were supported by spectra provided by a rapid scanning ultraviolet detector. Two extraction procedures were devel oped to isolate corticosteroids from both water-soluble and lipid-soluble s ample components. When applied to a variety of topical formulations, the tw o extraction methods eliminated most of the sample excipients, recovered co rticosteroids in high yield, and allowed for detection to a level of 0.001% of the sample.