Isolation of flavonoid aglycones from propolis by a column chromatography method and their identification by GC-MS and TLC methods

Authors
Citation
W. Maciejewicz, Isolation of flavonoid aglycones from propolis by a column chromatography method and their identification by GC-MS and TLC methods, J LIQ CHR R, 24(8), 2001, pp. 1171-1179
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
8
Year of publication
2001
Pages
1171 - 1179
Database
ISI
SICI code
1082-6076(2001)24:8<1171:IOFAFP>2.0.ZU;2-3
Abstract
Ten flavonoid compounds were isolated by means of preparative column chroma tography from propolis collected in southern Poland. Their mass spectra wer e taken and compared with a spectrum library. As a result, nine of them wer e identified as: tectochrysin, pinocembrin, chrysin, galangin, genkwanin, a pigenin, kaempferol, pilloin, 5-hydroxy-4',7-dimethoxyflavone, and pinostro bin chalcone. Pilloin and pinostrobin chalcone were isolated from propolis for the first time. The optimum system of solvents for separation on a silica gel column was chosen on the basis on R-F vs. mixed solvent composition dependence, ob tained by means of thin-layer chromatography.