A rapid HPLC-DAD method for separation and determination of omeprazole extracted from human plasma

Citation
Mc. Dubuc et al., A rapid HPLC-DAD method for separation and determination of omeprazole extracted from human plasma, J LIQ CHR R, 24(8), 2001, pp. 1161-1169
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
8
Year of publication
2001
Pages
1161 - 1169
Database
ISI
SICI code
1082-6076(2001)24:8<1161:ARHMFS>2.0.ZU;2-W
Abstract
A high-performance liquid chromatographic (HPLC) method for the determinati on of omeprazole in human plasma is described. Omeprazole and the internal standard (H168/24) were extracted from plasma samples by solid phase extrac tion (SPE) using a polymeric sorbent-based cartridge. The separation was ac complished under reversed phase conditions using an Eclipse XDB-C8 Rapid Re solution (4.6 x 50 mm) column. The mobile phase consisted of 23% acetonitrile and 77% of 30.4mM Na2HPO4 an d 1.76mM KH2PO4 solution, pH 8.4, in which a gradient elution was used to l inearly change solvent composition to 33% acetonitrile and 67% phosphate bu ffer during the first minute. Absorbance was monitored at 302 nm for omepra zole and at 294 nm for the internal standard and the total analysis time wa s 4 minutes. The lower limit of quantitation was 10 ng/mL and the calibration function i s linear to 2000ng/mL. This method has been shown to be appropriate for pha rmacokinetic studies involving children.