HPLC determination of the major factors of the nourseothricin complex, an antibiotic for nonmedical uses

Citation
K. Dornberger et al., HPLC determination of the major factors of the nourseothricin complex, an antibiotic for nonmedical uses, J LIQ CHR R, 24(8), 2001, pp. 1153-1159
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
8
Year of publication
2001
Pages
1153 - 1159
Database
ISI
SICI code
1082-6076(2001)24:8<1153:HDOTMF>2.0.ZU;2-Z
Abstract
A liquid chromatographic method has been developed for the quantification o f nourseothricin (NTC) in fermentation broths. The two major components D a nd F of the nourseothricin complex, were separated by HPLC using a C8 bond silica gel column and an aqueous ion pair solvent consisting of 0.02% octan e-1-sulfonic acid sodium salt, 0.05% trifluoroacetic acid and 43% acetonitr ile. The individual components, as well as unknown minor constituents, were dete cted by UV 210 nm. It was decided to quantify NTC based on the sum of the p eak areas of D and F. The analytical response was linear over a concentrati on range of 10-80 mug/mL.