Chiral capillary electrophoretic separation of selected drugs and metabolites using sulfated beta-cyclodextrin

Citation
Ps. Bonato et al., Chiral capillary electrophoretic separation of selected drugs and metabolites using sulfated beta-cyclodextrin, J LIQ CHR R, 24(8), 2001, pp. 1115-1131
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
8
Year of publication
2001
Pages
1115 - 1131
Database
ISI
SICI code
1082-6076(2001)24:8<1115:CCESOS>2.0.ZU;2-P
Abstract
In this paper, we report the chiral separation of some drugs and their meta bolites using sulfated beta -cyclodextrin, a powerful chiral selector that has proven to be highly efficient for the separation of chiral drugs, alone or in combination, with other chiral additives. Praziquantel, its metaboli te trans-4-hydroxypraziquantel, and albendazole sulfoxide are neutral compo unds and could be separated at basic pH (8-10). The simultaneous chiral separation of praziquantel and its metabolite enant iomers was only possible by the addition of sodium deoxycholate to the runn ing buffer. Fluoxetine, disopyramide, and mexiletine, as well as their meta bolites are basic compounds and were separated by appropriate selection of the running buffer pH and CD concentration. In addition, the effects of the experimental parameters, such as concentration of sulfated beta -cyclodext rin, pH and buffer concentration, temperature, and voltage were also evalua ted. Among the several parameters studied, the concentration of the chiral selec tor and the pH were the most important to obtain the chiral separation of t he selected drugs and metabolites.