In this paper, we report the chiral separation of some drugs and their meta
bolites using sulfated beta -cyclodextrin, a powerful chiral selector that
has proven to be highly efficient for the separation of chiral drugs, alone
or in combination, with other chiral additives. Praziquantel, its metaboli
te trans-4-hydroxypraziquantel, and albendazole sulfoxide are neutral compo
unds and could be separated at basic pH (8-10).
The simultaneous chiral separation of praziquantel and its metabolite enant
iomers was only possible by the addition of sodium deoxycholate to the runn
ing buffer. Fluoxetine, disopyramide, and mexiletine, as well as their meta
bolites are basic compounds and were separated by appropriate selection of
the running buffer pH and CD concentration. In addition, the effects of the
experimental parameters, such as concentration of sulfated beta -cyclodext
rin, pH and buffer concentration, temperature, and voltage were also evalua
Among the several parameters studied, the concentration of the chiral selec
tor and the pH were the most important to obtain the chiral separation of t
he selected drugs and metabolites.