Determination of alditols and carbohydrates of food interest using a sulfonated monodisperse resin-based column, coupled with pulsed amperometric detection (PAD) and postcolumn PH adjustment

Citation
C. Corradini et al., Determination of alditols and carbohydrates of food interest using a sulfonated monodisperse resin-based column, coupled with pulsed amperometric detection (PAD) and postcolumn PH adjustment, J LIQ CHR R, 24(8), 2001, pp. 1073-1088
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
8
Year of publication
2001
Pages
1073 - 1088
Database
ISI
SICI code
1082-6076(2001)24:8<1073:DOAACO>2.0.ZU;2-I
Abstract
Carbohydrates such as glucose, fructose, sucrose, and sugar alcohols mannit ol, sorbitol, and xylitol are widely distributed in various foods and bever ages. In this work we developed a HPLC method for the determination of sugars and alditols, using a sulfonated monodisperse resin-based column in hydrogen f orm (PL Hi-Plex, 300 x 7.7 mm, from Polymer Laboratories), coupled with pul sed amperometric detection (PAD). Performing isocratic separation using wat er as the mobile phase, post column addition of 0.5 M sodium hydroxide was necessary to detect sugars and alditols by PAD. After the optimization of t he operating conditions, the method was characterized and validated in term s of selectivity, repeatability, sensitivity, and accuracy. Furthermore, qu antification of lactose, glucose, and galactose in lactose-hydrolyzed milk products was found to be highly accurate.