Phylogenetic relationships among microsporidia based on rDNA sequence data, with particular reference to fish-infecting Microsporidium Balbiani 1884 species.

Citation
As. Bell et al., Phylogenetic relationships among microsporidia based on rDNA sequence data, with particular reference to fish-infecting Microsporidium Balbiani 1884 species., J EUKAR MIC, 48(3), 2001, pp. 258-265
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN journal
1066-5234 → ACNP
Volume
48
Issue
3
Year of publication
2001
Pages
258 - 265
Database
ISI
SICI code
1066-5234(200105/06)48:3<258:PRAMBO>2.0.ZU;2-5
Abstract
Recently. large discrepancies have bean identified between microsporidian s ystematics based on molecular and traditional characteristics. In the curre nt study the 530f-580r region of the rRNA gene of eight microsporidian spec ies was cloned and sequenced. included were two unclassified species of Mic rosporidium Balbiani. 1884 and an unidentified microsporidian that infects the musculature of different sea bream species. Sequence identities in exce ss of 98% indicated that these three species almost certainly are members o f the same genus, Phylogenetic analyses of all microsporidian sequence data available for this region of the gene (20 species) and for partial small s ubunit sequences (51 species of 21 general revealed these species to be dis tinct from the family Pleistophoridae Doflein. 1901 and closely related the m to the genus Spraguea Weissenberg, 1976. This clade was found to comprise a sister taxon to that containing the vast majority of` fish-infecting spe cies. Broad cladistic divisions were found between terrestrial insect-infec ting and fish-infecting species, which together are distant from the aquati c insect-infecting microsporidia. The rRNA gene of certain fish-infecting g enera was found to be more highly conserved than previously reported. This has implications for its utility in diagnostic assays and phylogenetic: stu dies at, or close to. the species level.