Phylogeny of Pneumocystis carinii from 18 primate species confirms host specificity and suggests coevolution

Citation
C. Demanche et al., Phylogeny of Pneumocystis carinii from 18 primate species confirms host specificity and suggests coevolution, J CLIN MICR, 39(6), 2001, pp. 2126-2133
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
6
Year of publication
2001
Pages
2126 - 2133
Database
ISI
SICI code
0095-1137(200106)39:6<2126:POPCF1>2.0.ZU;2-6
Abstract
Primates are regularly infected by fungal organisms identified as Pneumocys tis carinii. They constitute a valuable population for the confirmation of P. carinii host specificity. In this study, the presence of P, carinii was assessed by direct examination and nested PCR at mitochondrial large subuni t (mtLSU) rRNA and dihydropteroate synthetase (DHPS) genes in 98 lung tissu e samples from captive or wild nonhuman primates. Fifty-nine air samples co rresponding to the environment of different primate species in zoological p arks were also examined. Cystic forms of P. carinii were detected in smears from 7 lung tissue samples corresponding to 5 New World primate species. A mplifications at the mtLSU rRNA gene were positive for 29 lung tissue sampl es representing 18 different primate species or subspecies and 2 air sample s corresponding to the environment of two simian colonies. Amplifications a t the DHPS gene were positive for 8 lung tissue samples representing 6 diff erent primate species. Direct sequencing of nested PCR products demonstrate d that a specific mtLSU rRNA and DHPS sequence could be attributed to each primate species or subspecies. No nonhuman primate harbored the human type of P. carinii (P. carinii f. sp. hominis). Genetic divergence in primate-de rived P. carinii organisms varied in terms of the phylogenetic divergence e xisting among the corresponding host species, suggesting coevolution.