Cell cycle regulation by galectin-12, a new member of the galectin superfamily

Citation
Ry. Yang et al., Cell cycle regulation by galectin-12, a new member of the galectin superfamily, J BIOL CHEM, 276(23), 2001, pp. 20252-20260
Citations number
75
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
276
Issue
23
Year of publication
2001
Pages
20252 - 20260
Database
ISI
SICI code
0021-9258(20010608)276:23<20252:CCRBGA>2.0.ZU;2-3
Abstract
Galectins are a family of beta -galactoside-binding animal lectins with con served carbohydrate recognition domains (CRDs). Here we report the identifi cation and characterization of a new galectin, galectin-12, which contains two domains that are homologous to the galectin CRD. The N-terminal domain contains all of the sequence elements predicted to form the two beta -sheet s found in other galectins, as well as conserved carbohydrate-interacting r esidues. The C-terminal domain shows considerable divergence from the conse nsus sequence, and many of these conserved residues are not present. Nevert heless, the protein has lactose binding activity, most Likely due to the co ntribution of the N-terminal domain. The mRNA for galectin-12 contains feat ures coding for proteins with growth-regulatory functions. These include st art codons in a context that are suboptimal for translation initiation and AU-rich motifs in the 3'-untranslated region, which are known to confer ins tability to mRNA. Galectin-12 mRNA is sparingly expressed or undetectable i n many tissues and cell lines tested, but it is up-regulated in cells synch ronized at the G(1) phase or the G(1)/S boundary of the cell cycle. Ectopic expression of galectin-12 in cancer cells causes cell cycle arrest at the G(1) phase and cell growth suppression. We conclude that galectin-12 is a n ovel regulator of cellular homeostasis.