Induction of CD4(+) regulatory T cells by 12-O-tetradecanoylphorbol 13-acetate in mice Part 1: The strain difference in this induction

Citation
T. Negoro et al., Induction of CD4(+) regulatory T cells by 12-O-tetradecanoylphorbol 13-acetate in mice Part 1: The strain difference in this induction, INT IMMUNO, 1(6), 2001, pp. 1153-1163
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL IMMUNOPHARMACOLOGY
ISSN journal
1567-5769 → ACNP
Volume
1
Issue
6
Year of publication
2001
Pages
1153 - 1163
Database
ISI
SICI code
1567-5769(200106)1:6<1153:IOCRTC>2.0.ZU;2-P
Abstract
12-O-Tetradecanoylphorbol 13-acetate (TPA), an activator of signal transduc tion, induced antigen-nonspecific regulatory T (Tr) cells for delayed-type hypersensitivity (DTH) in the spleen. A marked strain difference in the ind uction of Tr cells was observed when the study was performed by using the s everal strains of mice at 6-8 weeks old. TPA painting induced CD4(+)Tr cell s in C3H/He (H-2(k)), C3H/HeN (H-2(k)), DBA/2 (H-2(d)) and AKR/N (H-2(k)) m ice, but not in C57BL/6 (H-2(b)), C57BL/10 (H-2(b)), BALB/c (H-2(d)) and A/ J (H-2(a)). Regulatory cells were also induced by incubating spleen cells from unprimed mice with TPA in vitro and were seemed to act by the production of soluble factor(s). A downregulatory activity of the soluble factor(s) was abrogate d by SXC-1 (anti-IL-10 monoclonal antibody), but not by SXC-2 (anti-IL-10 m onoclonal antibody) and anti-IL-4. For purification of the factor(s), we established the T cell hybridoma 4C5- 1 by the fusion of spleen cells from TPA-treated C3H/He mice with AKR thymo ma Bw5147 cells. The 4C5-1 cells secrete the factor(s) which can inhibit DT H response. The inhibitory activity of the factor(s) could be neutralized b y SXC-1, but not by SXC-2, anti-IL-4, anti-IL-6 and anti-TGF-beta antibodie s. The factor(s) could not affect the proliferation and IFN-gamma productio n of alpha s1-casein-specific 3D20 Th1 cells. The factor(s) termed DIF (DTH Inhibitory Factor) may be a novel cytokine, since they have reduced the fo otpad swelling response by local injection, and have no immune crossreactiv ity with the DTH regulatory cytokines and no inhibitory activity for in vit ro Th1 response. (C) 2001 Elsevier Science B.V. All rights reserved.