Antioxidant defense systems of two lipidopteran insect cell lines

Citation
Y. Wang et al., Antioxidant defense systems of two lipidopteran insect cell lines, FREE RAD B, 30(11), 2001, pp. 1254-1262
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN journal
0891-5849 → ACNP
Volume
30
Issue
11
Year of publication
2001
Pages
1254 - 1262
Database
ISI
SICI code
0891-5849(20010601)30:11<1254:ADSOTL>2.0.ZU;2-C
Abstract
Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5BI-4 (Tn-5BI- 4) insect cell lines were found to contain unique assemblages of antioxidan t enzymes. Specifically, the Sf-9 insect cell line contained Manganese and Copper-Zinc superoxide dismutase (MnSOD and CuZnSOD) for reducing the super oxide radical (O-2(.-)) to hydrogen peroxide (H2O2) and ascorbate peroxidas e (APOX) for reducing the resulting H2O2 to H2O. Approximately one third of the total SOD activity was found to be MnSOD. The Tn-5BI-4 cells were also found to contain MnSOD (similar to two thirds of the total SOD activity), CuZnSOD and APOX activities. However, the Tn-5B1-4 cell line, in contrast t o the Sf-9 cell line, contained catalase (CAT) activity for reducing H2O2 t o H2O. Both the Sf-9 and Tn-SB1-4 cell lines contained glutathione reductas e and dehydroascorbic acid reductase activities for regenerating the reduce d forms of glutathione and ascorbic acid, respectively. In addition, both c ell lines contained glutathione S-transferase peroxidase activity towards h ydroperoxides other than H2O2. Finally, neither cell line contains the glut athione peroxidase activity that is ubiquitous in mammalian cells. (C) 2001 Elsevier Science Inc.