Injection of CD4(+) and CD8(+) cells with donor or host accessory cells induces acute graft-vs-host disease in human skin in immunodeficient mice

Citation
M. Matsumoto et al., Injection of CD4(+) and CD8(+) cells with donor or host accessory cells induces acute graft-vs-host disease in human skin in immunodeficient mice, EXP HEMATOL, 29(6), 2001, pp. 720-727
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
EXPERIMENTAL HEMATOLOGY
ISSN journal
0301-472X → ACNP
Volume
29
Issue
6
Year of publication
2001
Pages
720 - 727
Database
ISI
SICI code
0301-472X(200106)29:6<720:IOCACC>2.0.ZU;2-H
Abstract
Objective. We examined cell subsets with respect to cutaneous graft-vs-host disease by cell sorting selection of subsets of human mononuclear cells an d injecting the subsets subcutaneously in a mouse model. Materials and Methods. Cell suspensions containing cultured human epidermal cells and dermal fibroblasts from a single donor mixed with lymphoid cell subsets positively selected using the FACSVantage cell sorting instrument a nd/or MACS cell isolation kits from unrelated individuals were injected int o immunodeficient mice, This model is known to generate human skin with his tologic findings similar to human graft-vs-host disease. Results, Donor T-cell subsets CD4(+) and CD8(+) plus either host or donor C D14(+) cells were necessary to cause acute cutaneous graft-vs-host disease. Although graft-vs-host disease can result from recognition of class P anti gens expressed on human cutaneous cells by donor peripheral blood mononucle ar cells, additional recognition of class II antigens expressed on host mon onuclear cells resulted in more severe histologic manifestations. Dendritic cells that differentiated from donor and host monocytes also showed compet ent accessory cell function in this system. Conclusions. Based on this model, human cutaneous graft-vs-host disease was caused by donor CD4(+) cells and CD8+ cells activated through recognition of host antigens, including class I and class II antigens presented by eith er donor or host CD14(+) cells or dendritic cells. (C) 2001 International S ociety for Experimental Hematology, Published by Elsevier Science Inc.