NF-kappa B activation in peripheral blood monocytes/macrophages and T cells during acute Kawasaki disease

Citation
T. Ichiyama et al., NF-kappa B activation in peripheral blood monocytes/macrophages and T cells during acute Kawasaki disease, CLIN IMMUNO, 99(3), 2001, pp. 373-377
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
CLINICAL IMMUNOLOGY
ISSN journal
1521-6616 → ACNP
Volume
99
Issue
3
Year of publication
2001
Pages
373 - 377
Database
ISI
SICI code
1521-6616(200106)99:3<373:NBAIPB>2.0.ZU;2-3
Abstract
Kawasaki disease (KD) is a febrile disease of childhood characterized by sy stemic vasculitis, and the levels of many proinflammatory cytokines are ele vated in the serum at the acute stage. We investigated the activation of tr anscription factor NF-kappaB for genes that encode the proinflammatory cyto kines in CD14(+) monocytes/macrophages and CD3(+) T cells in peripheral blo od by means of Western blot and flow cytometric analyses. Western. blot ana lysis demonstrated that NF-kappaB activation was more increased in CD14(+) monocytes/macrophages than in CD3(+) T cells in all children during the acu te stage. Flow cytometric analysis revealed that NF-kappaB activation in CD 14(+) monocytes/macrophages was significantly higher than in CD3(+) T cells at the acute stage (30.0 +/- 16.0% vs 11.4 +/- 5.0%, P < 0.01, Wilcoxon te st). NF-kappaB activation in CD14(+) mono-cytes/macrophages was significant ly decreased after high-dose intravenous immunoglobulin therapy (P < 0.05). The present findings suggest that CD14(+) monocyte/macrophages play an imp ortant role in cytokine production during acute KD. (C) 2001 Academic Press .