Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload

Medvedev, A Adamik, A Glover, V Telegdy, G

A. Medvedev et al., Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload, BIOCH PHARM, 62(2), 2001, pp. 225-227

16

INGLESE

Article

Pharmacology & Toxicology

BIOCHEMICAL PHARMACOLOGY

0006-2952 → ACNP

62

2

2001

225 - 227

ISI

0006-2952(20010715)62:2<225:CGEBBI>2.0.ZU;2-P

Isatin is a potent inhibitor of atrial natriuretic peptide (ANP) receptors and ANP-induced generation of cGMP in vitro. This study was designed to det ermine whether it had a similar effect in vivo, using a model of fluid over load known to induce ANP. We confirmed that this model increased urinary ou tput of cGMP 3 hr after volume loading, and showed that this effect was blo cked by i.p. injection of isatin (50 mg/kg). Isatin had no effect on urine volume or sodium output. However, isatin did have an effect on plasma prote in concentration, both compared with control values, compatible with shifti ng fluid to the vascular compartment, and after volume overload, in which i t normalised such a shift. Isatin thus affected both the generation of cGMP and fluid balance in vivo. (C) 2001 Elsevier Science Inc. All rights reser ved.