Postsynaptic modulation of AMPA receptor-mediated synaptic responses and LTP by the type 3 ryanodine receptor

Citation
M. Shimuta et al., Postsynaptic modulation of AMPA receptor-mediated synaptic responses and LTP by the type 3 ryanodine receptor, MOL CELL NE, 17(5), 2001, pp. 921-930
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
MOLECULAR AND CELLULAR NEUROSCIENCE
ISSN journal
1044-7431 → ACNP
Volume
17
Issue
5
Year of publication
2001
Pages
921 - 930
Database
ISI
SICI code
1044-7431(200105)17:5<921:PMOARS>2.0.ZU;2-2
Abstract
The precise function of ryanodine receptors (RyRs) in synaptic transmission is unknown, but three of their subtypes are expressed in the brain. We exa mined the role of RyRs in excitatory synaptic transmission in hippocampal s lices, using type 3 RyR (RyR3)-deficient mice. The alpha -amino-3-hydroxy-5 -methyl-4-isoxo-zolepropionic acid (AMPA) receptor-mediated basal synaptic responses in the CA1 region of mutant mice were smaller than those of wild- type mice, while there was no difference in N-methyl-D-aspartate receptor-m ediated responses, suggesting selective postsynaptic modification of AMPA r eceptors by RyR3. The expression of synaptic AMPA receptor subunits examine d by Western blotting or immunohistochemistry was indistinguishable, sugges ting that the smaller AMPA synaptic responses in mutant mice were not due t o the reduced number of synaptic AMPA receptors. Although the initial poten tiation following tetanic stimulation of afferent fibers was similar, long- term potentiation (LTP) was smaller in mutant mice. There were no differenc es in presynaptic electrophysiological properties. We thus conclude that Ry R3 postsynaptically regulates the properties of AMPA receptors and LTP.