Determination of polyamines in beers by HPTLC with fluorescence detection

Citation
Rm. Linares et al., Determination of polyamines in beers by HPTLC with fluorescence detection, J PL CHROM, 14(1), 2001, pp. 4-7
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
ISSN journal
0933-4173 → ACNP
Volume
14
Issue
1
Year of publication
2001
Pages
4 - 7
Database
ISI
SICI code
0933-4173(200101/02)14:1<4:DOPIBB>2.0.ZU;2-C
Abstract
A high-performance thin-layer chromatographic (HPTLC) method has been devel oped for determination of polyamines in beers. Beer samples, previously deg assed, were derivatized with dansyl chloride and the dansyl derivatives mer e separated by HPTLC with silica gel as stationary phase and chloroform-tri ethylamine, 2 + 1 (v/v), containing 5% (m/v) polyoxyethylene-10-lauryl ethe r, as mobile phase. Quantitative analysis was achieved by in-Situ fiberopti c-based fluorescence scanning. The compounds were determined over the range 0.5-85 ng, with relative standard deviations between 0.44-1.16% and detect ion limits in the range 0.28-0.39 ng. The simple preparation of the sample and the rapid microwave-assisted dansylation considerably reduce analysis t ime and effort.