Plasma levels of interlenkin-6 and interleukin-10 in preterm neonates evaluated for sepsis

C. Romagnoli et al., Plasma levels of interlenkin-6 and interleukin-10 in preterm neonates evaluated for sepsis, EUR J PED, 160(6), 2001, pp. 345-350
Citations number
Categorie Soggetti
Pediatrics,"Medical Research General Topics
Journal title
ISSN journal
0340-6199 → ACNP
Year of publication
345 - 350
SICI code
In a prospective study, plasma interleukin-6 (IL-6) and interleukin-10 (IL- 10) levels were measured by enzyme-linked immunosorbent assay in 45 prematu re neonates (25-34 weeks gestational age) with signs and symptoms of suspec ted sepsis at 0, 12 and 24 h; C-reactive protein (CRP) was measured at 0-24 h after enrolment. Six subjects were excluded due to insufficient blood sa mpling. The remaining 39 neonates were assigned to one of three groups: 25 newborns with sepsis (blood culture positive), seven with pneumonia (positi ve results on broncho-alveolar lavage fluid culture and characteristic ches t radiography) and seven with necrotising enterocolitis (NEC) (characterist ic intestinal and radiological signs according to the criteria of Bell et a l.). A group of 20 healthy preterm neonates represented control subjects. O n admission, higher levels of IL-6, IL-10 and CRP were observed in neonates with sepsis: IL-6 (median 1500 pg/ml, range 487-10000 pg/ml), IL-10 (media n 113 pg/ml, range 70-196 pg/ml), CRP (median 22 mg/l, range 4-80 mg/l); pn eumonia: IL-6 (median 1500 pg/ml, range 747-8000 pg/ml, IL-10 (median 84 pg /ml, range 76-92 pg/ml), CRP (median 10 mg/l, range 8-33 mg/l) and NEC: IL- 6 (median 6650 pg/ml, range 1595-7950 pg/ml), IL-10 (median 80 pg/ml, range 61-147 pg/ml), CRP (median 3 mg/l, range 2.8-8 mg/l) as compared to contro ls (IL-6 median 208 pg/ml, range 198-349 pg/ml; IL-10 median 36 pg/ml, rang e 19-50 pg/ml; CRP median <2 mg/l) (P < 0.05). In neonates with sepsis, IL- 6 levels were significantly correlated with IL-10 levels (r = 0.65; P = 0.0 4) at the time of the second sample. The highest IL-6 levels were observed at onset, while IL-10 was predominant 12 h later. On admission, IL-10 and C RP levels were significantly higher in non-survivors (IL-10 median 507 pg/m l, range 422-753 pg/ml; CRP median 123 mg/l, range 20-219 mg/l) than in sur vivors (IL-10 median 76 pg/ml, range 61-143 pg/ml; CRP median 8 mg/l range 3-46 mg/l), while IL-10 levels were significantly higher(P < 0.05) also 12 h after admission (non-survivors: IL-10 median 600 pg/ml, range 538-800 pg/ ml; survivors: IL-10 median 74 pg/ml, range 53-161 pg/ml). IL-6 and IL-10 l evels were significantly correlated with CRP levels on admission (r = 0.45; P = 0.05). Conclusion Preterm neonates with sepsis, pneumonia or necrotising enterocol itis showed increased interleukin-6, interleukin-10 and C-reactive protein levels. High interleukin-10 concentration was associated with mortality and could be an early indicator of prognosis.