Identification of dual specificity phosphatases induced by olfactory bulbectomy in rat olfactory neuroepithelium

Citation
T. Ishibashi et al., Identification of dual specificity phosphatases induced by olfactory bulbectomy in rat olfactory neuroepithelium, BRAIN RES, 902(2), 2001, pp. 205-211
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
0006-8993 → ACNP
Volume
902
Issue
2
Year of publication
2001
Pages
205 - 211
Database
ISI
SICI code
0006-8993(20010601)902:2<205:IODSPI>2.0.ZU;2-4
Abstract
Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase/stress-activated pr otein kinase (JNK/SAPK), and p38 mitogen-activated protein kinases (MAPKs) which are target enzymes activated by a wide range of cell-surface stimuli. Like these kinases, a class of dsPTP has been implicated in cell different iation, regeneration, and apoptosis. In order to isolate dsPTPs which might play an important role in neuronal regeneration and apoptosis in olfactory neuroepithelium, we subcloned DNA fragments amplified by reverse transcrip tion-polymerase chain reaction (RT-PCR), using degenerate oligonucleotide p rimers based on the conserved amino acid regions within the catalytic domai n of dsPTPs, from rat olfactory epithelial RNA 1 and 4 h after an olfactory bulbectomy. The PCR products were subcloned into the pCRII vector, and 23 clones were chosen for further characterization. The sequence of these 23 i ndividual clones revealed that two clones were identical to the rat dsPTP, MKP-3, and the other 21 clones were identical to the rat dsPTP, MKP-1. By N orthern analysis, the MKP-1 transcript was induced and peaked 4 h following a bulbectomy. Similar results were obtained with the MKP-3 transcript. The se results suggest that MKP-1 and MKP-3 may be involved in the early steps of apoptosis in vivo in rat olfactory neuroepithelium. (C) 2001 Elsevier Sc ience B.V. All rights reserved.