Defibrinogenating effect of batroxobin (Defibrase (R)) in rats and inhibition of migration of human vascular smooth muscle cells by the plasma of batroxobin-treated rats in vitro

Citation
Ds. Wang et al., Defibrinogenating effect of batroxobin (Defibrase (R)) in rats and inhibition of migration of human vascular smooth muscle cells by the plasma of batroxobin-treated rats in vitro, ATHEROSCLER, 156(1), 2001, pp. 73-80
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Journal title
ATHEROSCLEROSIS
ISSN journal
0021-9150 → ACNP
Volume
156
Issue
1
Year of publication
2001
Pages
73 - 80
Database
ISI
SICI code
0021-9150(200105)156:1<73:DEOB((>2.0.ZU;2-H
Abstract
The defibrinogenating effect of batroxobin (Defibrase(R)) in male Wistar ra ts and the inhibitory effects of the plasma of batroxobin-treated rats on t he migration of human vascular smooth muscle cells (SMCs) were investigated in vitro. At 1 h after a single intravenous injection of 3.0, 10.0 or 30.0 BU/kg batroxobin (ten rats in each group), the fibrinogen levels in the pl asma of the rats decreased to 88.3, 66.2 and 16.5%. respectively. of that i n the plasma of control saline-treated rats (261.0 +/- 26.7 mg/dl). When th e plasma from the batroxobin-treated rats was added to Dulbecco's modified Eagle's medium at a concentration of 0.2% for a vascular SMC migration assa y and incubated in a modified Boyden's chamber system at 37 degreesC for 24 h, significant inhibitory effects on vascular SMC migration were observed in the 10.0 (P < 0.05) and 30.0 BU/kg (P < 0.01) batroxobin-treated rats. T he plasma of batroxobin-treated rats as well as standard rat fibrinogen ind uced vascular SMC migration in a fibrinogen content-dependent manner except the plasma of the 30.0 BU/kg batroxobin-treated rats. Moreover, the rat se rum (0.1 similar to 5.0%) did not show any activity on vascular SMC migrati on in the present experimental system. These results indicate that the plas ma fibrinogen significantly influences vascular SMC migration, and that the inhibitory effect of the plasma of batroxobin-treated rats on vascular SMC migration is related to the defibrinogenating action of batroxobin in vivo . (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.