Genetic and functional characterization of dpp genes encoding a dipeptide transport system in Lactococcus lactis

Citation
Y. Sanz et al., Genetic and functional characterization of dpp genes encoding a dipeptide transport system in Lactococcus lactis, ARCH MICROB, 175(5), 2001, pp. 334-343
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
175
Issue
5
Year of publication
2001
Pages
334 - 343
Database
ISI
SICI code
0302-8933(200105)175:5<334:GAFCOD>2.0.ZU;2-H
Abstract
The genes encoding a binding-protein-dependent ABC transporter for dipeptid es (Dpp) were identified in Lactococcus lactis subsp. cremoris MG1363. Two (dppA and dppP) of the six ORFs (dppAdpPBCDF) encode proteins that are homo logous to peptide- and pheromone-binding proteins. The dppP gene contains a chain-terminating nonsense mutation and a frame-shift that may impair its function. The functionality of the dpp genes was proven by the construction of disruption mutants via homologous recombination. The expression of DppA and various other components of the proteolytic system was studied in synt hetic and peptide-rich media and by using isogenic peptide-transport mutant s that are defective in one or more systems (Opp, DtpT and/or Dpp). In pept ide-rich medium, DppA was maximally expressed in mutants lacking Opp and Dt pT. DppA expression also depended on the growth phase and was repressed by tri-leucine and tri-valine. The effect of tri-leucine on DppA expression wa s abolished when leucine was present in the medium. Importantly, the Dpp sy stem also regulated the expression of other components of the proteolytic s ystem. This regulation was achieved via the internalization of di-valine, w hich caused a 30-50% inhibition in the expression of the proteinase PrtP an d the peptidases PepN and PepC. Similar to the regulation of DppA, the repr essing effect was no longer observed when high concentrations of valine wer e present. The intricate regulation of the components of the proteolytic sy stem by peptides and amino acids is discussed in the light of the new and p ublished data.