Cytotoxic activity of saponins from Camassia leichtlinii against human oral tumor cell lines

Citation
S. Furuya et al., Cytotoxic activity of saponins from Camassia leichtlinii against human oral tumor cell lines, ANTICANC R, 21(2A), 2001, pp. 959-964
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ANTICANCER RESEARCH
ISSN journal
0250-7005 → ACNP
Volume
21
Issue
2A
Year of publication
2001
Pages
959 - 964
Database
ISI
SICI code
0250-7005(200103/04)21:2A<959:CAOSFC>2.0.ZU;2-F
Abstract
Five steroidal saponins from Camassia leichtlinii showed higher cytotoxicit y against human oral squamous cell carcinoma cells HSC-2, as compared to no rmal human gingival fibroblasts HCF. The rumor specificity of saponins vari ed considerably from sample to sample, but was generally higher than that o f tannins, flavonoids and prenylated compounds such as geranylgeraniol and vitamin K-2 (MK-2). Agarose gel electrophoresis showed that the saponins fa iled to induce internucleosomal DNA fragmentation, but produced large DNA f ragments in HSC-2 cells, whereas two saponin samples (compounds I and 5) in duced internucleosomal DNA fragmentation in human promyelocytic leukemic HL -60 cells. In contrast to epigallocatechin gallate or gallic acid, the cyto toxic activity of saponins was not significantly affected by metals (Co2+, Cu2+, Fe3+) or by antioxidants (sodium ascorbate, N-acetyl-L-cysteine, cata lase). Furthermore, the saponins did not produce radicals (detected by ESR spectroscopy) nor oxidation potential (measured by NO monitor). These data suggest that an oxidation-mediated mechanism is not involved in the cytotox icity induced by the steroidal saponins.