Isolation and sequence of the MIG1 homologue from the yeast Candida utilis

Citation
J. Delfin et al., Isolation and sequence of the MIG1 homologue from the yeast Candida utilis, YEAST, 18(7), 2001, pp. 597-603
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749-503X → ACNP
Volume
18
Issue
7
Year of publication
2001
Pages
597 - 603
Database
ISI
SICI code
0749-503X(200105)18:7<597:IASOTM>2.0.ZU;2-6
Abstract
The Mig1p repressor from the food yeast Candida utilis has been isolated us ing a homologous PCR hybridization probe. This probe was amplified with two sets of degenerate primers designed on the basis of highly conserved motif s in the DNA-binding region (zinc-finger domain) from yeast Mig1p and fungi CreA repressors. The cloned gene was sequenced and found to encode a polyp eptide of 345 amino acids which shows significant identity with other yeast and fungus repressors in the DNA-binding domain and also with the yeast Mi g1 proteins in the C-terminal region (effector domain). The MIG1 repressor gene from C. utilis was able to complement functionally the mig1 mutation o f S. cerevisiae. The sequence presented here has been deposited in the EMBL data library under Accession No. AJ277830. Copyright (C) 2001 John Wiley & Sons, Ltd.