Domoic acid accumulation in French shellfish in relation to toxic species of Pseudo-nitzschia multiseries and P-pseudodelicatissima

Citation
Z. Amzil et al., Domoic acid accumulation in French shellfish in relation to toxic species of Pseudo-nitzschia multiseries and P-pseudodelicatissima, TOXICON, 39(8), 2001, pp. 1245-1251
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICON
ISSN journal
0041-0101 → ACNP
Volume
39
Issue
8
Year of publication
2001
Pages
1245 - 1251
Database
ISI
SICI code
0041-0101(200108)39:8<1245:DAAIFS>2.0.ZU;2-6
Abstract
Within the French phytoplankton monitoring network (REPHY), domoic acid (DA ), the toxin responsible for amnesic shellfish poisoning, was first detecte d in samples collected in 1998. Toxin analysis by the official method [liqu id chromatography with diode array detection (LC/DAD)] was performed when P seudo-nitzschia cell concentration was greater than 1.0 X 10(5) cells/l. LC /DAD results obtained in 1999 and 2000 showed increased DA accumulation in bivalves sampled at different sites along French coasts. The toxin maximum in 1999 was 3.2 mug DA/g of whole tissue, whereas the levels in 2000 (53 mu g) were above the sanitary threshold (20 mug DA/g tissue). Phytoplankton sa mples collected during blooms were observed by both light and scanning elec tron microscopy (SEM). Identification of phytoplankton species by SEM analy ses confirmed the presence of two known DA-producing species. P. pseudodeli catissima and P. multiseries. LC/DAD results for a mass culture of P. multi series indicated that this species: was involved in DA accumulation in Fren ch shellfish. (C) 2001 Elsevier Science Ltd. All rights reserved.