An efficient method for the cryopreservation of fetal human liver hematopoeitic progenitor cells

Citation
J. Zhao et al., An efficient method for the cryopreservation of fetal human liver hematopoeitic progenitor cells, STEM CELLS, 19(3), 2001, pp. 212-218
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
STEM CELLS
ISSN journal
1066-5099 → ACNP
Volume
19
Issue
3
Year of publication
2001
Pages
212 - 218
Database
ISI
SICI code
1066-5099(2001)19:3<212:AEMFTC>2.0.ZU;2-0
Abstract
The use of human hematopoietic progenitor cells (HPC) for transplantation r equires efficient recovery methods and cryopreservation procedures. The pur pose of this study was to determine cryopreservation techniques for fetal h uman liver (FHL) CD34(+) cells. We assessed FHL HPC recovery efficiency aft er freezing and thawing by viability testing, fluoresrence-activated cell s orting analysis, and colony-forming ability under different conditions. We also determined optimal cell freezing concentrations and the effect of rate -controlled freezing on cell recovery. Lastly, cell recovery after varying freezing time periods was examined. Our results indicated that optimal cell recovery occurs when: A) cryopreservation medium consists of either 5% dim ethylsulphoxide (DMSO) or 10% DMSO in combination with either 20% fetal bov ine serum (FBS) or 70% FBS and when Iscove's modified Dulbecco's medium con sists of not more than 10% DMSO; B) a rate-controlled freezing device conta iner is used; C) CD34(+) cells are frozen at a concentration of 1 x 10(6)/m l, and D) a thawing temperature of 37 degreesC is used, These observations indicate that cryopreservation of FHL HPC is possible for up to 18 months i n optimal conditions without losing hematopoietic activity.