Determination of free D-aspartic acid, D-serine and D-alanine in the brainof mutant mice lacking D-amino-acid oxidase activity

Citation
A. Morikawa et al., Determination of free D-aspartic acid, D-serine and D-alanine in the brainof mutant mice lacking D-amino-acid oxidase activity, J CHROMAT B, 757(1), 2001, pp. 119-125
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
757
Issue
1
Year of publication
2001
Pages
119 - 125
Database
ISI
SICI code
1387-2273(20010605)757:1<119:DOFDAD>2.0.ZU;2-7
Abstract
A simple and precise method for the simultaneous determination of free D-as partic acid, D-serine and D-alanine in mouse brain tissues was established, using a reversed-phase HPLC system with widely used pre-column derivatizin g reagents, o-phthaldialdehyde and N-t-butyloxycarbonyl-L-cysteine. With th e present method, the contents of these three D-amino acids in hippocampus. hypothalamus, pituitary gland, pineal gland and medulla oblongata as well as cerebrum and cerebellum of mutant mice lacking D-amino-acid oxidase acti vity were determined and compared with those obtained for control mice. In both mice, extremely high contents of D-serine were observed in forebrain ( 100-400 nmol/g wet tissue), and the contents were small in pituitary and pi neal glands. While, D-serine contents in cerebellum and medulla oblongata o f mutant mice were about ten times higher than those in control mice. In co ntrast, D-alanine contents in mutant mice were higher than those in control mice in all brain regions and serum. (C) 2001 Elsevier Science B.V. All ri ghts reserved.