Quality control of PCR primers used in multiplex STR amplification reactions

Citation
Jm. Butler et al., Quality control of PCR primers used in multiplex STR amplification reactions, FOREN SCI I, 119(1), 2001, pp. 87-96
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
FORENSIC SCIENCE INTERNATIONAL
ISSN journal
0379-0738 → ACNP
Volume
119
Issue
1
Year of publication
2001
Pages
87 - 96
Database
ISI
SICI code
0379-0738(20010601)119:1<87:QCOPPU>2.0.ZU;2-Y
Abstract
Reliable amplification of short tandem repeat (STR) DNA markers with the po lymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification reactions where multiple STR loci are simulta neously copied. Commercially available kits are now widely used for STR amp lification and subsequent DNA typing. We present here the use of high perfo rmance liquid chromatography (HPLC) and time-of-flight mass spectrometry (T OF-MS) methods for characterization of commercially available STR kits. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.