The phosphocholine motif in membranes of Mycoplasma fermentans strains

Citation
G. Ben-menachem et al., The phosphocholine motif in membranes of Mycoplasma fermentans strains, FEMS MICROB, 199(1), 2001, pp. 137-141
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
0378-1097 → ACNP
Volume
199
Issue
1
Year of publication
2001
Pages
137 - 141
Database
ISI
SICI code
0378-1097(20010515)199:1<137:TPMIMO>2.0.ZU;2-P
Abstract
Mycoplasma fermentans strains differ in the profile of choline-containing p hosphoglycolipids (PGL) present in their cell membrane. MfGL-II [Zahringer et al. (1997) J. Biol. Chem. 272, 26262-76270] was found to be the major PG L in most strains tested. However, in the pulmonary isolates, M52 and M39 t he major choline-containing PGLs were MfGL-1 [Matsuda et al. (1993) J. Biol . Chem. 269, 33123-33129] and MfEL. a unique choline-containing ether lipid recently identified by us [Wagner et al. (2000) fur. J. Biochem. 267, 6276 6286]. Both MfGL-I and MfEL were metabolically labeled by growing the cell s with radioactive choline but only MfGL-I reacted with anti-phosphocholine antibodies. All tested strains fused with Molt-3 cells at almost the same rate and to about the same extent and in all the strains membrane proteins that reacted with anti-phosphocholine antibodies were detected. indicating that some membrane proteins are decorated with phosphocholine moieties. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of Eur opean Microbiological Societies.