Black tea extract, thearubigin fraction, counteracts the effect of tetanustoxin in mice

Citation
E. Satoh et al., Black tea extract, thearubigin fraction, counteracts the effect of tetanustoxin in mice, EXP BIOL ME, 226(6), 2001, pp. 577-580
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
EXPERIMENTAL BIOLOGY AND MEDICINE
ISSN journal
1535-3702 → ACNP
Volume
226
Issue
6
Year of publication
2001
Pages
577 - 580
Database
ISI
SICI code
1535-3702(200106)226:6<577:BTETFC>2.0.ZU;2-F
Abstract
The aim of this study was to find an inactivating substance for tetanus tox in in natural foodstuff. Tetanus toxin (4 mug/ml) abolished indirect twitch es in in vitro mouse phrenic nerve-diaphragm preparations within 2.5 hr. Ho t water infusion of black tea mixed with tetanus toxin blocked the inhibito ry effect of the toxin. Mixing the toxin with thearubigin fraction extracte d from black tea infusion produced an identical result, Furthermore, thearu bigin fraction mixed with the toxin protected against the in vivo paralytic effect of the toxin. Thearubigin fraction had no protective effect on othe r toxins, such as tetrodotoxin and saxitoxin. The specific binding of [I-12 5]tetanus toxin to rat cerebrocortical synaptosomes was inhibited by mixing iodinated toxin with thearubigin fraction. These results imply that thearu bigin fraction counteracts the effect of tetanus toxin by binding with toxi n, and also suggest that this fraction may be able to apply for prophylaxis of tetanus.