Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites

Citation
Y. Suzuki et al., Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites, EMBO REP, 2(5), 2001, pp. 388-393
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO REPORTS
ISSN journal
1469-221X → ACNP
Volume
2
Issue
5
Year of publication
2001
Pages
388 - 393
Database
ISI
SICI code
1469-221X(200105)2:5<388:DTIRBF>2.0.ZU;2-N
Abstract
Determination of the mRNA start site is the first step in identifying the p romoter region, which is of key importance for transcriptional regulation o f gene expression. The 'oligo-capping' method enabled us to introduce a seq uence tag to the first base of an mRNA by replacing the cap structure of th e mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identif y the transcriptional start site of an individual mRNA just by sequencing t he 5'-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation o f 19.5. Our finding may reflect the dynamic nature of transcriptional initi ation events of human genes in vivo.