Preparation of levoglucosan by pyrolysis of cellulose and its citric acid fermentation

Citation
Xl. Zhuang et al., Preparation of levoglucosan by pyrolysis of cellulose and its citric acid fermentation, BIORES TECH, 79(1), 2001, pp. 63-66
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIORESOURCE TECHNOLOGY
ISSN journal
0960-8524 → ACNP
Volume
79
Issue
1
Year of publication
2001
Pages
63 - 66
Database
ISI
SICI code
0960-8524(200108)79:1<63:POLBPO>2.0.ZU;2-B
Abstract
Levoglucosan (LG), 1,6-anhydro-beta -D-glucopyranose. was produced by pyrol ysis of cellulose. A response surface method was used to optimize the react ion parameters: X-1, temperature; X-2, time required for heating cellulose from room temperature to the designed pyrolysis temperature; and X-3. vacuu m, and a Box-Behnken design was employed for this purpose. The optimal temp erature and time were found to be 388 degreesC and 26.2 min by fixing the v acuum at 1 mm HE. The levoglucosan prepared was fermented to citric acid by Aspergillus niger CBX-309, which was a mutant derived by gamma -ray mutage nesis of the parent strain CBX-2. The mutant could produce increasing citri c acid with increasing LG purity and had a citric acid yield of 87.5% when using purified levoglucosan as the sole carbon source in a 5 day fermentati on period. (C) 2001 Elsevier Science Ltd. All rights reserved.