A novel in vivo P-31-nuclear magnetic resonance technique for assessment of liver failure induced by chlorinated-hydrocarbon in mice

Citation
N. Manabe et al., A novel in vivo P-31-nuclear magnetic resonance technique for assessment of liver failure induced by chlorinated-hydrocarbon in mice, BIOMED RES, 21(6), 2000, pp. 317-326
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
BIOMEDICAL RESEARCH-TOKYO
ISSN journal
0388-6107 → ACNP
Volume
21
Issue
6
Year of publication
2000
Pages
317 - 326
Database
ISI
SICI code
0388-6107(200012)21:6<317:ANIVPM>2.0.ZU;2-F
Abstract
Novel in vivo P-31-, H-1- and H-2-nuclear magnetic resonance (NMR) techniqu es for accurate and noninvasive assessment of hepatic failure induced by ch lorinated-hydrocarbons, major environmental toxicants found in tap water, w ere developed. Male mice were administered halocarbons (trichloromethane, t etrachloromethane, tetrachloroethane; 0.0001-1,000 mug/kg of body weight on ce or daily for 4 weeks; p.o.). In vivo NMR spectra acquired noninvasively and quantitatively were used to evaluate their hepatic energy metabolism an d blood flow rate, and liver failure was estimated by conventional blood bi ochemical and histopathological methods. At doses of 0.1-1,000 mug/kg, a si gnificant decrease in liver ATP level was seen 0.5 h after administration a nd continued to drop showing maximum reduction between 1-2 h and recovered by 120 h. However, no changes in serum transaminase activities or liver his topathology were seen at doses of 1 or 10 mug/kg. In mice with repeated adm inistration (0.0001-1 mug/kg), no liver failure was detected by conventiona l biochemical or histopathological methods, but consistent decreases in the hepatic ATP level and liver blood flow rate were detected by the NMR techn iques. In conclusion, in vivo NMR techniques are highly sensitive (at least 100-fold more sensitive than conventional methods) and are useful for toxi cological assessment of environmental pollutants in tap water.