Distinct electrophoretic isozyme profiles of Fusarium graminearum and closely related species

Citation
M. Laday et A. Szecsi, Distinct electrophoretic isozyme profiles of Fusarium graminearum and closely related species, SYST APPL M, 24(1), 2001, pp. 67-75
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
0723-2020 → ACNP
Volume
24
Issue
1
Year of publication
2001
Pages
67 - 75
Database
ISI
SICI code
0723-2020(200104)24:1<67:DEIPOF>2.0.ZU;2-W
Abstract
Cellulose-acetate electrophoresis (CAE) was used to investigate isozyme pol ymorphisms among different isolates of Fusarium cerealis, F. culmorum, F. g raminearum and F. pseudograminearum from around the world. After initial te sting of 22 enzymes in three buffer systems for activity and resolution of bands, 12 proved to be appropriate for analysis of the full sample set. Rem arkably uniform isozyme patterns were obtained intraspecifically, irrespect ive of the geographical origin of the isolates or the host/substratum from which they were isolated. This result indicated that isolates within a give n species are descendant from a same ancestral population. Comparing the di fferent electrophoretic types (ETs), adenylate kinase (AI(), NADP dependent glutamate dehydrogenase (NADP GDH), peptidase B (PEP B), peptidase D (PEP D) and phosphoglucomutase (PGM) proved to be diagnostic for at least one sp ecies examined. However, only PEP D was useful alone as a marker to disting uish the four taxa studied providing a rapid and simple CAE based diagnosti c protocol. Cluster analysis of band sharing coefficients grouped the isola tes into four distinct groups corresponding to the 4 species studied. Isola tes of F. cerealis were clustered between those of F. culmorum and F, grami nearum corroborating their known close relationship to both species. For co mmon ETs, the similarity values between F. cerealis and F. culmorum and bet ween F. cerealis and F. graminearum were the same. Furthermore, the similar ity values and the resulting phenogram indicated that F. graminearum is mor e closely related to E: cerealis and F. culmorum than to F. pseudograminear um, thus the morphological similarity of F. graminearum and F. pseudogramin earum does not reflect their genetic relationship. This fact supports the s pecies status of F. pseudograminearum.