Effects of deflected droplet electrostatic cell sorting on the viability and exoproteolytic activity of bacterial cultures and marine bacterioplankton

Citation
O. Resina-pelfort et al., Effects of deflected droplet electrostatic cell sorting on the viability and exoproteolytic activity of bacterial cultures and marine bacterioplankton, SYST APPL M, 24(1), 2001, pp. 31-36
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
0723-2020 → ACNP
Volume
24
Issue
1
Year of publication
2001
Pages
31 - 36
Database
ISI
SICI code
0723-2020(200104)24:1<31:EODDEC>2.0.ZU;2-2
Abstract
The cell-sorting capability of flow cytometers makes it possible to isolate specific populations of cells with pre-defined cytometric characteristics. A better knowledge of the biological effects of the sorting process is nec essary for the future cell sorting applications. In this paper we report th e effects of flow cy tometric sorting on bacterial viability and exoproteol ytic activity (EPA) of bacterial cultures and marine bacterioplankton. Sort ing bacterial cultures and bacterioplankton samples reduce viability as ass essed by plate counts and produce variations in the exoproteolytic activity . These effects indicate that deflected electrostatic sorting may significa ntly alter the biological properties of the sorted bacteria.