Stimulation of saccharifying enzyme production by immobilized fungal mycelia using liquefied rice treated with protease

Citation
S. Shindo et S. Takahashi, Stimulation of saccharifying enzyme production by immobilized fungal mycelia using liquefied rice treated with protease, J BIOSCI BI, 91(3), 2001, pp. 256-261
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
91
Issue
3
Year of publication
2001
Pages
256 - 261
Database
ISI
SICI code
1389-1723(200103)91:3<256:SOSEPB>2.0.ZU;2-1
Abstract
The effects of protease-treated liquefied rice on the production of sacchar ifying enzyme by immobilized fungal mycelia were investigated. The producti on of saccharifying enzyme by immobilized fungal mycelia was stimulated by the protease-treated liquefied rice. The origin of the effector was identif ied as a hydrolyzate of glutelin, a major rice protein. Moreover, the effec tor was purified by gel permeation chromatography and reversed-phase HPLC, The molecular weight of the purified effector was around 400-500 Da and it was composed of a few amino acids. These results indicate that the oligopep tide derived from the glutelin hydrolyzate regulates the production of sacc harifying enzyme by immobilized fungal mycelia. Furthermore, this oligopept ide stimulated the production of raw starch-digesting glucoamylase by immob ilized fungal mycelia but did not stimulate the production of other protein s.