Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture

Citation
N. Sasayama et al., Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture, CYTOTHERAPY, 3(2), 2001, pp. 117-126
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
Journal title
CYTOTHERAPY
ISSN journal
1465-3249 → ACNP
Volume
3
Issue
2
Year of publication
2001
Pages
117 - 126
Database
ISI
SICI code
1465-3249(2001)3:2<117:EOMPFC>2.0.ZU;2-#
Abstract
Long-term severe thrombocytopenia following human placental and umbilical c ord blood (CB) transplantation is a significant clinical problem. We studie d the ex vivo expansion of megakaryocytic progenitor cells (CFU-Meg) from c ryopreserved/thawed leukocyte concentrates (LC) of CB prepared by the Tokyo Cord Blood Bank protocol. The LC cells were cultured in serum-free culture medium supplemented with a combination of early-acting cytokines including thrombopoietin (TPO), flt3-ligand (FL), and stem cell factor (SCF). Combin ation of TPO plus FL, TPO plus SCF, and all of these cytokines together res ulted in 8.9-, 7.7-, and 8.4-fold increases in CFU-Meg, respectively, by Da y 5 of culture. Our results showed that this simple expansion strategy has the potential for expanding CFU-Meg from cryopreserved/thawed LC cells from CB.