Hippuric acid test using C-13-Labelling and NMR spectroscopy

Citation
K. Akira et T. Hashimoto, Hippuric acid test using C-13-Labelling and NMR spectroscopy, CLIN CH L M, 39(3), 2001, pp. 215-217
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
ISSN journal
1434-6621 → ACNP
Volume
39
Issue
3
Year of publication
2001
Pages
215 - 217
Database
ISI
SICI code
1434-6621(200103)39:3<215:HATUCA>2.0.ZU;2-C
Abstract
This article describes a C-13-labelling and nuclear magnetic resonance appr oach for hippuric acid test which is potentially useful for evaluating live r reserve. In this approach, urine samples collected after ingestion of C-1 3-labelled benzoic acid were directly analysed by C-13 nuclear magnetic res onance spectroscopy, and the excreted C-13-labelled hippuric acid formed fr om the administered benzoic acid was quantitated. The amount of labelled hi ppuric acid excreted in a specified time can be a useful index of liver res erve. In this study, the feasibility of the nuclear magnetic resonance appr oach has been investigated in several healthy subjects. This approach is si mple and convenient compared with conventional analytical procedures, becau se no chromatographic separation is required. The approach could give new i nsights into the liver reserve, because the benzoic acid conversion to hipp uric acid intimately relates to the hepatic energy metabolism. This measure ment can be conducted at a wide range of dosages without interference from endogenous hippuric acid.