MtDNA from extinct Tainos and the peopling of the Caribbean

Citation
C. Lalueza-fox et al., MtDNA from extinct Tainos and the peopling of the Caribbean, ANN HUM GEN, 65, 2001, pp. 137-151
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
ANNALS OF HUMAN GENETICS
ISSN journal
0003-4800 → ACNP
Volume
65
Year of publication
2001
Part
2
Pages
137 - 151
Database
ISI
SICI code
0003-4800(200103)65:<137:MFETAT>2.0.ZU;2-H
Abstract
Tainos and Caribs were the inhabitants of the Caribbean when Columbus reach ed the Americas; both human groups became extinct soon after contact, decim ated by the Spaniards and the diseases they brought. Samples belonging to p re-Columbian Taino Indians from the La Caleta site (Dominican Republic) hav e been analyzed, in order to ascertain the genetic affinities of these grou ps in relation to present-day Amerinds, and to reconstruct the genetic and demographic events that took place during the peopling of the Caribbean. Twenty-seven bone samples were extracted and analyzed for mtDNA variation. The four major Amerindian mtDNA lineages were screened through amplificatio n of the specific marker regions and restriction enzymatic digestion, when needed. The HVRI of the control region was amplified with four sets of over lapping primers and sequenced in 19 of the samples. Both restriction enzyme and sequencing results suggest that only two (C and D) of the major mtDNA lineages were present in the sample: 18 individuals (75%) belonged to the C haplogroup, and 6 (25%) to the D haplogroup. Sequences display specific su bstitutions that are known to correlate with each haplogroup, a fact that h elped to reject the possibility of European DNA contamination. A low rate o f Taq misincorporations due to template damage was estimated from the cloni ng and sequencing of different PCR products of one of the samples. High fre quencies of C and D haplogroup, are more common in South American populatio ns, a fact points to that sub-continent as the homeland of the Taino ancest ors, as previously suggested by linguistic and archaeological evidence. Seq uence and haplogroup data show that the Tainos had a substantially reduced mtDNA diversity, which is indicative of an important founder effect during the colonization of the Caribbean Islands, assumed to have been a linear mi gratory movement from mainland South America following the chain configurat ion of the Antilles.