Single-cell sorting of microorganisms by flow or slide-based (including laser scanning) cytometry

Citation
T. Katsuragi et Y. Tani, Single-cell sorting of microorganisms by flow or slide-based (including laser scanning) cytometry, ACT BIOTECH, 21(2), 2001, pp. 99-115
Citations number
63
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
ACTA BIOTECHNOLOGICA
ISSN journal
0138-4988 → ACNP
Volume
21
Issue
2
Year of publication
2001
Pages
99 - 115
Database
ISI
SICI code
0138-4988(2001)21:2<99:SSOMBF>2.0.ZU;2-3
Abstract
In applied microbiology, strain improvement of microorganisms by convention al selection culture is not always successful, so single-cell selection of viable cells with the desired characteristics from a large heterogeneous po pulation may be used instead. Single-cell selection with use of a micromani pulator is possible, but laborious. For many applications, the process has been automated. In this review, an automated method, laser scanning cytomet ry (LSC), is outlined together with flow cytometry (FCM). FCM is familiar t o many microbiologists. but LSC is a microscopic-slide-based method that is less well known. One of its advantages is its possible use in the examinat ion of small cell populations. In addition, individual cells can be examine d repeatedly. measured automatically and later observed microscopically by the operator, and finally stood (if desired) on the microscopic slide on wh ich they are placed. Fluorescent and other probes an available in abundance for FCR I. and almost all might be used in LSC. A number of applications o f these methods are cited from the extensive literature (mostly about FCM), but the list of possible applications in this review is far from being exh austive. This review is intended as an introduction for the applied microbi ologist to the manifold uses of LSC.