Ma. Kuhn et al., Basic fibroblast growth factor in a carboxymethylcellulose vehicle reverses the bacterial retardation of wound contraction, WOUNDS, 13(2), 2001, pp. 73-80
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dermatology
Journal title
WOUNDS-A COMPENDIUM OF CLINICAL RESEARCH AND PRACTICE
Chronic granulating wounds containing greater than 10(5) bacteria/gram of t
issue of Escherichia coli (E. coli) were established on rats. Recombinant h
uman basic fibroblast growth factor (bFGF) in a carboxymethylcellulose (CMC
) vehicle was applied at dosages of 1, 10, and 100 mug/cm(2) to the wounds
of three groups of five animals on days 5, 9, 12, 15, and 18 after injury.
The rate of wound closure was compared to that of similarly wounded animals
treated with CMC vehicle alone and wounded animals that were neither treat
ed with vehicle nor infected. High levels of bacteria caused significant im
pairment of wound contraction. Addition of bFGF at all concentrations (1, 1
0, and 100 mug/cm(2)) markedly improved the rate of wound closure while ine
rt vehicle applied alone was ineffective. Since bacterial counts did not de
crease in the bFGF-treated wounds, bFGF was not inherently bactericidal. Hi
stological examination of the bFGF-treated wounds showed increased cellular
ity with increased number of fibroblasts and round cells. These results con
firm findings that bFGF can overcome the defect in healing created by bacte
rial infection, and this peptide may have efficacy in the management of the
contaminated wound. The CMC vehicle acts to release the bFGF over a sustai
ned period and protects the cytokine against wound-associated proteases mak
ing this vehicle superior to previously reported bFGF formulations. By prov
iding a concentration gradient of longer duration, the concentration of the
growth factor needed to effectively improve the rate of wound closure is d
ecreased.