Basic fibroblast growth factor in a carboxymethylcellulose vehicle reverses the bacterial retardation of wound contraction

Citation
Ma. Kuhn et al., Basic fibroblast growth factor in a carboxymethylcellulose vehicle reverses the bacterial retardation of wound contraction, WOUNDS, 13(2), 2001, pp. 73-80
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dermatology
Journal title
WOUNDS-A COMPENDIUM OF CLINICAL RESEARCH AND PRACTICE
ISSN journal
1044-7946 → ACNP
Volume
13
Issue
2
Year of publication
2001
Pages
73 - 80
Database
ISI
SICI code
1044-7946(200103/04)13:2<73:BFGFIA>2.0.ZU;2-Z
Abstract
Chronic granulating wounds containing greater than 10(5) bacteria/gram of t issue of Escherichia coli (E. coli) were established on rats. Recombinant h uman basic fibroblast growth factor (bFGF) in a carboxymethylcellulose (CMC ) vehicle was applied at dosages of 1, 10, and 100 mug/cm(2) to the wounds of three groups of five animals on days 5, 9, 12, 15, and 18 after injury. The rate of wound closure was compared to that of similarly wounded animals treated with CMC vehicle alone and wounded animals that were neither treat ed with vehicle nor infected. High levels of bacteria caused significant im pairment of wound contraction. Addition of bFGF at all concentrations (1, 1 0, and 100 mug/cm(2)) markedly improved the rate of wound closure while ine rt vehicle applied alone was ineffective. Since bacterial counts did not de crease in the bFGF-treated wounds, bFGF was not inherently bactericidal. Hi stological examination of the bFGF-treated wounds showed increased cellular ity with increased number of fibroblasts and round cells. These results con firm findings that bFGF can overcome the defect in healing created by bacte rial infection, and this peptide may have efficacy in the management of the contaminated wound. The CMC vehicle acts to release the bFGF over a sustai ned period and protects the cytokine against wound-associated proteases mak ing this vehicle superior to previously reported bFGF formulations. By prov iding a concentration gradient of longer duration, the concentration of the growth factor needed to effectively improve the rate of wound closure is d ecreased.