Protective immunity to Schistosoma japonicum infection depends on the balance of T helper cytokine responses in mice vaccinated with gamma-irradiatedcercariae

Citation
Y. Osada et al., Protective immunity to Schistosoma japonicum infection depends on the balance of T helper cytokine responses in mice vaccinated with gamma-irradiatedcercariae, PARASITE IM, 23(5), 2001, pp. 251-258
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
PARASITE IMMUNOLOGY
ISSN journal
0141-9838 → ACNP
Volume
23
Issue
5
Year of publication
2001
Pages
251 - 258
Database
ISI
SICI code
0141-9838(200105)23:5<251:PITSJI>2.0.ZU;2-J
Abstract
Although the strain difference in protection of mice to Schistosoma mansoni infection has been described, limited information is available in the case of Schistosoma japonicum In the present study, we compared the protective immunity to S. japonicum infection and cytokine production in various strai ns of mice vaccinated with gamma -irradiated cercariae. A significant reduc tion in worm recovery was observed in male and female mice of DBA/2 at a 6- week interval between vaccination and a challenge infection, whereas vaccin ated mice of C57BL/6, C57BL/10, (C57BL/6 x DBA/2) F1 (B6D2F1) and (C57BL/ 1 0 x DBA/2) FI (B10D2F1) showed no detectable level of protection. No sex-li nked difference in development of resistance was observed in any of the str ains so far examined. Vaccination with gamma -irradiated cercariae twice wi th a 3-week interval also induced significant protection against a challeng e infection in DBA/2 but not in BALB/c or C57BL/6 strains. Further studies demonstrated that spleen cells of vaccinated C57BL/6 mice produced lower le vels of IFN-gamma compared to the cells of vaccinated BALB/c and DBA/2. On the other hand, production of IL-10 by spleen cells was relatively higher i n BALB/c mice than in the other two strains. Macrophages that had been stim ulated with spleen cell culture supernatants derived from vaccinated DBA/2 damaged schistosomula more effectively than cells stimulated with supernata nts derived from the other strains. These results suggest that different le vels of protection observed among strains of mice depend on the balance of cytokine responses which consequently activate or suppress macrophage-media ted damage to schistosomula.