Regulation of the PH-domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain

Citation
Ry. Chen et al., Regulation of the PH-domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain, NAT CELL BI, 3(5), 2001, pp. 439-444
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
NATURE CELL BIOLOGY
ISSN journal
1465-7392 → ACNP
Volume
3
Issue
5
Year of publication
2001
Pages
439 - 444
Database
ISI
SICI code
1465-7392(200105)3:5<439:ROTPTK>2.0.ZU;2-5
Abstract
Etk/BMX, a member of the Btk family of tyrosine kinases, is highly expresse d in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines. Here, we present evidence that Etk is invo lved in integrin signalling and promotes cell migration. The activation of Etk by extracellular matrix proteins is regulated by FAK through an interac tion between the PH domain of Etk and the FERM domain of FAK. The lack of E tk activation by extracellular matrix in FAK-null cells could be restored b y co-transfection with wild-type FAK, Disrupting the interaction between Et k and FAK diminished the cell migration promoted by either kinase. Furtherm ore, inhibiting Etk expression in metastatic carcinoma cell lines with an a ntisense oligonucleotide blocks integrin-mediated migration of these cells. Taken together, our data indicate the essential role of the interaction of the PH domain of Etk and the FERM domain of FAK in integrin signalling.