Sequencing and amplified restriction fragment length polymorphism analysisof ribonucleotide reductase large subunit gene of the white spot syndrome virus in blue crab (Callinectes sapidus) from American coastal waters

Citation
Ys. Chang et al., Sequencing and amplified restriction fragment length polymorphism analysisof ribonucleotide reductase large subunit gene of the white spot syndrome virus in blue crab (Callinectes sapidus) from American coastal waters, MAR BIOTEC, 3(2), 2001, pp. 163-171
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
MARINE BIOTECHNOLOGY
ISSN journal
1436-2228 → ACNP
Volume
3
Issue
2
Year of publication
2001
Pages
163 - 171
Database
ISI
SICI code
1436-2228(200103/04)3:2<163:SAARFL>2.0.ZU;2-#
Abstract
In the present study, the existence of white spot syndrome virus (WSSV) in blue crab (Callinectes sapidus) collected from 3 different American coastal waters (New York, New Jersey, and Texas) was confirmed by 2-step diagnosti c polymerase chain reaction and in situ hybridization analysis. When geogra phic isolates were also compared using a gene that encodes the WSSV ribonuc leotide reductase large subunit RR1 (WSSV rr1), a C-1661-to-T point mutatio n was found in the New Jersey WSSV isolated. This point mutation, which res ulted in the creation of an additional RsaI endonuclease recognition site, was not found in the WSSV from the New York and Texas blue crab samples, or in the WSSV Taiwan isolate, or in any of the other WSSV geographical isola tes for which data are available. WSSV rrl-specific RsaI amplified restrict ion fragment length polymorphism of an amplified 1156-bp fragment thus dist inguished the New Jersey blue crab samples from the other WSSV isolates.