Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum)

Citation
Tj. Yoon et al., Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum), INT IMMUNO, 1(5), 2001, pp. 881-889
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL IMMUNOPHARMACOLOGY
ISSN journal
1567-5769 → ACNP
Volume
1
Issue
5
Year of publication
2001
Pages
881 - 889
Database
ISI
SICI code
1567-5769(200105)1:5<881:CAHAAO>2.0.ZU;2-C
Abstract
The adjuvant effect of lectins (KML-C) isolated from Korean mistletoe (Visc um album coloratum) on induction of humoral and cellular immune responses a gainst keyhole limpet hemocyanine (KLH) was examined. When mice were immuni zed subcutaneously (s.c.) with KLH (20 mug/mouse) admired with or without 5 0 ng/mouse of KML-C (KLH + KML-C), mice immunized with KLH + KML-C showed s ignificantly higher antibody titers against KLH than those immunized with K LH alone. showing the highest titer 5 weeks after immunization. Furthermore , boost immunization with KLH + KML-C at 2-week interval elicited much high er activity than single immunization to enhance antibody responses against KLH. The assay for determining isotypes of antibodies revealed that KML-C a ugmented KLH-specific antibody titers of IgG1, IgG2a and IgG2b. The culture supernatants obtained from the splenocytes of mice treated with KLH + KML- C also showed a higher level of both KLH-specific Th-l (IL-2 and IFN-gamma) and Th-2 type cytokine (IL-1). In an in vitro analysis of T lymphocyte pro liferation to KLH on week 4, the splenocytes of mice treated with KLH + KML -C showed a significantly higher proliferating activity than those treated with KLH alone, In addition, mice immunized twice with KLH + KML-C and foll owed by intrafootpad (i.f.) injection of KLH (50 mug/site) 14 weeks after t he primary immunization induced a higher delayed-type hypersensitivity (DTH ) reaction than mice treated with KLH alone. These results suggest that KML -C is a potent immunoadjuvant to enhance cellular and humoral immune respon ses. (C) 2001 Elsevier Science B.V. All nights reserved.