Regulation of gene expression, cellular localization, and in vivo functionof Caenorhabditis elegans DNA topoisomerase I

Citation
Mh. Lee et al., Regulation of gene expression, cellular localization, and in vivo functionof Caenorhabditis elegans DNA topoisomerase I, GENES CELLS, 6(4), 2001, pp. 303-312
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENES TO CELLS
ISSN journal
1356-9597 → ACNP
Volume
6
Issue
4
Year of publication
2001
Pages
303 - 312
Database
ISI
SICI code
1356-9597(200104)6:4<303:ROGECL>2.0.ZU;2-N
Abstract
Background: DNA topoisomerase I is dispensable in yeast, but is essential d uring the embryogenesis of Drosophila and mouse. In order to determine func tions of the enzyme in the development of Caenorhabditis elegans, phenotype s resulting from the deficiency were observed and correlated with the expre ssion of the gene. Results: The transcriptional regulation of the C. elegans DNA topoisomerase I gene was investigated by mRNA localization and reporter gene expression in C. elegans. The mRNA was expressed in the gonad and in the early embryos , followed by a rapid decrease in its level during the late embryonic stage . A reporter gene expression induced by the 5'-upstream DNA sequence appear ed at the comma stage of embryos, continued through the L1 larval stage, an d began to decrease gradually afterwards. The DNA topoisomerase I protein w as immuno-localized in the nuclei of meiotic gonad cells and interphase emb ryonic cells, and unexpectedly in centrosomes of mitotic embryonic cells. D ouble-stranded RNA interference of DNA topoisomerase I gene expression resu lted in pleiotropic phenotypes showing abnormal gonadogenesis, oocyte devel opment and embryogenesis. Conclusion: These phenotypes, along with expressional regulations, demonstr ate that DNA topoisomerase I plays important roles in rapidly growing germ cells and embryonic cells.