Distribution of aminoglycoside resistance genes in recent clinical isolates of Enterococcus faecalis, Enterococcus faecium and Enterococcus avium

Citation
N. Kobayashi et al., Distribution of aminoglycoside resistance genes in recent clinical isolates of Enterococcus faecalis, Enterococcus faecium and Enterococcus avium, EPIDEM INFE, 126(2), 2001, pp. 197-204
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
EPIDEMIOLOGY AND INFECTION
ISSN journal
0950-2688 → ACNP
Volume
126
Issue
2
Year of publication
2001
Pages
197 - 204
Database
ISI
SICI code
0950-2688(200104)126:2<197:DOARGI>2.0.ZU;2-H
Abstract
Aminoglycoside modifying enzymes (AMEs) are major factors which confer amin oglycoside resistance on bacteria. Distribution of genes encoding seven AME s was investigated by multiplex PCR for 279 recent clinical isolates of ent erococci derived from a university hospital in Japan. The aac(6')-aph(2"), which is related to high level gentamicin resistance, was detected at highe r frequency in Enterococcus faecalis (42.5 %) than in Enterococcus faecium (4.3 %). Almost half of E. faecalis and E. faecium isolates possessed ant(6 )-Ia and aph(3')-IIIa. The profile of AME gene(s) detected most frequently in individual strains of E. faecalis was aac(6')-aph(2")+ ant(6)-Ia + aph(3 ')-IIIa, and isolates with this profile showed high level resistance to bot h gentamicin and streptomycin. In contrast, AME gene profiles of aac (6')-I i+ ant(6)-Ia+aph(3')-IIIa, followed by aac(6')-Ii alone, were predominant i n E. faecium. Only one AME gene profile of ant(6)-Ia + aph(3')-IIIa was fou nd in Enterococcus avium. The ant(4')-Ia and ant(9)-Ia, which have been kno wn to be distributed mostly among Staphylococcus aureus strains, were detec ted in a few enterococcal strains. An AME gene aph(2")-Ic was not detected in any isolates of the three enterococcal species. These findings indicated a variety of distribution profiles of AME genes among enterococci in our s tudy site.