Inhibition of oligo(glutamine) precipitation by glutamine-containing peptides

Citation
Bcf. Chu et Le. Orgel, Inhibition of oligo(glutamine) precipitation by glutamine-containing peptides, BIOC BIOP R, 283(2), 2001, pp. 351-355
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
283
Issue
2
Year of publication
2001
Pages
351 - 355
Database
ISI
SICI code
0006-291X(20010504)283:2<351:IOOPBG>2.0.ZU;2-P
Abstract
When a solution of glutamine is added to solid 1,1'-carbonyldiimidazole, a mixture of oligo(glutamine)s up to about the 11-mer is formed rapidly. On s tanding overnight, the solution deposits a precipitate that does not easily redissolve. We have studied the inhibition of this precipitation by glutam ine-containing peptides. We find that the alternating peptides (arg.gln)(4) and (arg.gln)(8) are efficient inhibitors of precipitation while arg(5), ( glu.gln)(4), and a nonalternating octapeptide of the same composition as (a rg.gln)(4) do not inhibit precipitation even though all the arg-containing peptides readily adsorb to oligo(glutamine) precipitates. A possible struct ural basis for this difference is discussed. (C) 2001 Academic Press.