Photoacoustic measurement of ethylene as a real time biomarker of lipid peroxidation processes in mice

Citation
S. Stolik et al., Photoacoustic measurement of ethylene as a real time biomarker of lipid peroxidation processes in mice, ANAL SCI, 17, 2001, pp. S365-S367
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL SCIENCES
ISSN journal
0910-6340 → ACNP
Volume
17
Year of publication
2001
Pages
S365 - S367
Database
ISI
SICI code
0910-6340(2001)17:<S365:PMOEAA>2.0.ZU;2-D
Abstract
In some diseases the accumulated endogenous delta -aminolevulinic acid (del ta -ALA), with a pH between 7 and 8, undergoes an enolization process that produces free radicals. It is expected that most of the exogenously-supplie d GALA in healthy organisms will be converted into protoporphyrin IX (PpIX) , and ultimately to the hem group, but a small accumulation of delta -ALA, when present, will initiate a lipid peroxidation process due to the generat ion of oxygen-reactive species. Some of the end products commonly found in lipid peroxidation include ethane, pentane, and ethylene as well as other h ydrocarbons. As these gases are normally produced at trace levels, sensitiv e techniques are needed for their measurement. In this work, the peroxidati ve effect of exogenously-supplied GALA and CCl4 in female CD1 mice has been studied by photoacoustic trace gas detection. Exhaled ethylene from delta -ALA-administered mice reached concentrations of few ppbV for approximately 30 minutes, after application of delta -ALA.