Simple and rapid detection method using surface plasmon resonance for dioxins, polychlorinated biphenylx and atrazine

Citation
M. Shimomura et al., Simple and rapid detection method using surface plasmon resonance for dioxins, polychlorinated biphenylx and atrazine, ANALYT CHIM, 434(2), 2001, pp. 223-230
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
0003-2670 → ACNP
Volume
434
Issue
2
Year of publication
2001
Pages
223 - 230
Database
ISI
SICI code
0003-2670(20010511)434:2<223:SARDMU>2.0.ZU;2-E
Abstract
Endocrine disrupting chemicals (EDCs) such as dioxins, PCBs and pesticides are very harmful to human. In this work, a rapid detection method for 2,3,7 ,8-TCDD, PCB, and atrazine is described, which is based upon a competitive immunoassay using surface plasmon resonance (SPR). With this method, it was possible to detect 2,3,7,8-TCDD, PCB, and atrazine with respective detecti on limits of 0.1, 2.5, and 5 ng ml(-1), requiring 15 min for a single sampl e measurement. To achieve better sensitivities, a sandwich assay was additi onally performed. For dioxins and atrazine, response amplification could be observed, enabling better differentiation of small concentration differenc es among analytes. (C) 2001 Elsevier Science B.V. All rights reserved.